13. May 2022 | Application Areas
ZnO nanoparticles against biofilm and bacteria
Zinc oxide reduced into nanosized exhibits antibacterial activity due to the interaction of ZnO with the cell surface and inside of the bacteria making them adaptive candidate for antibacterial activity. ZnO nanoparticles (ZnO NPs) synthesised using plant extract and their biomolecules makes them a safer, non-toxic and bactericidal solution against pathogenic bacteria. Till date, only few research articles are published on analysing the antibiofilm and anti-virulence activity of ZnO NPs synthesised using Ruellia tuberosa extract. A new study was now focused on synthesising ZnO NPs using Ruellia tuberosa and efficacy to inhibit biofilm formation and virulence factor of Serratia marcescens.
Efficacy of green fabricated ZnO NPs
ZnO NPs at the concentration of 100 μg/mL showed highest antibacterial activity by reducing S. marcescens growth upto 4-fold in comparison to untreated bacteria (1.8 OD600nm). ZnO NPs also showed 50% of inhibition of biofilm formation 25 μg/mL concentration. Microscopic analysis revealed the phenotypical change in the biofilm matrix formation after treating with >25 μg/mL of ZnO NPs. Prodigiosin, a quorum sensing regulated factor of S. marcescens was also inhibited >70% at 100 μg/mL concentration of ZnO NPs. Therefore, the present study shows the efficacy of green fabricated ZnO NPs to inhibit the growth, biofilm, and virulence factor of S. marcescens.
The study has been published in Progress in Organic Coatings, Volume 166, May 2022.
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